Fig. 7: SETD5 inhibits adipogenesis in vivo. | Nature Communications

Fig. 7: SETD5 inhibits adipogenesis in vivo.

From: Spatiotemporal dynamics of SETD5-containing NCoR–HDAC3 complex determines enhancer activation for adipogenesis

Fig. 7

a Transplantation of 3T3-L1 preadipocytes into nude mice. 3T3-L1 preadipocytes transduced with control empty virus or SETD5-V5 were cultured for two days with MDI cocktail. Subsequently, harvested cells were implanted with HydroMatrix and injected into the subcutaneous region on the upper or lower back of nude mice. b, c Two weeks after transplantation, mice were dissected, and isolated transplanted fats were subjected to haematoxylin and eosin (H&E) staining (b) and immunohistochemistry using anti-perilipin-1 antibody (c). Representative images of transplants from the lower back are shown. d Quantitation of perilipin-1 positive adipocytes. Data are mean ± SE in the section of transplants from empty virus transduced (n = 5) or SETD5 transduced (n = 3) preadipocytes Unpaired two-tailed Student’s t-test. **p < 0.01. Source data are provided as a Source data file.

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