Fig. 3: Aphids are attracted to Y-sat-infected plants and produce more alate adults.
From: A plant virus satellite RNA directly accelerates wing formation in its insect vector for spread
All the statistical analysis in Fig. 3 was performed using χ2 test. a Mean percentages (±SE) of aphids attracted to CMV-infected, [CMV + Y-sat]-infected or healthy N. tabacum at 1 and 2 h after aphid transfer. CMV-infected vs [CMV + Y-sat]-infected (1 h, n = 127, P = 0.0005; 2 h, n = 126, P < 0.0001), and healthy vs [CMV + Y-sat]-infected (1 h, P = 0.0007; 2 h, P = 0.0093). b Mean percentages (±SE) of aphids attracted to CMV-infected, [CMV + Y-sat]-infected or healthy N. tabacum at 1 and 2 h after aphid transfer under dark conditions. For statistics, χ2 test was used. CMV-infected vs [CMV + Y-sat]-infected (1 h, n = 180, choice vs no choice, P < 0.0001; CMV vs [CMV + Y-sat], P = 0.715) (2 h, n = 180, choice vs no choice, P < 0.0001; CMV vs [CMV + Y-sat], P = 0.4652). c, d Number of aphids attracted to volatile compounds from [CMV + Y-sat]-infected, CMV-infected or healthy Nicotiana species in Y-tube choice experiment. c N. tabacum [CMV + Y-sat]-infected vs CMV-infected (n = 49, P = 0.8273); [CMV + Y-sat]-infected vs healthy (n = 44, P = 0.593); healthy vs CMV-infected (n = 40, P = 0.5637). d N. benthamiana [CMV + Y-sat]-infected vs CMV-infected (n = 34, P = 0.6171); [CMV + Y-sat]-infected vs healthy (n = 37, P = 0.593); healthy vs CMV-infected (n = 39, P = 0.8084). For the statistical significance (a–d), the symbols **, ***, **** are significance levels of P < 0.01, P < 0.001 and P < 0.0001, respectively. ns, not significant. e Mean percentages of alate and apterous morphs on healthy (n = 1316), CMV-infected (n = 750) and [CMV + Y-sat]-infected (n = 1521) N. tabacum. Statistical analysis for three samples was performed using χ2 test (three samples, P < 0.0001; [CMV + Y-sat] vs CMV, P < 0.0001; [CMV + Y-sat] vs healthy, P < 0.0001; CMV vs healthy, P < 0.0001). f Mean percentages of alate and apterous morphs of aphid populations infesting wild-type (n = 215) or transgenic (n = 133) N. benthamiana plants expressing Y-sat dsRNA (dsY-sat). Statistical analysis for two samples was performed using χ2 test (two samples, P = 0.0007). The results of the χ2 tests in Fig. 3 were also confirmed by another statistical method, a hypothesis test for one-sample (or two-sample) proportion in R (see the data set in Supplementary Methods 1). Source data are provided as a Source Data file.
