Fig. 6: Imaging of endogenous l-lactate release from glioblastoma cells.
From: A genetically encoded fluorescent biosensor for extracellular l-lactate
a Schematic representation of l-lactate metabolism in the presence of 25 mM of extracellular glucose. MCT monocarboxylate transporter, LDH lactate dehydrogenase. b Representative fluorescence images of T98G cells expressing eLACCO1.1 or deLACCO treated with 25 mM glucose. Scale bars, 20 μm. c ΔF/F versus time for T98G cells expressing eLACCO1.1 or deLACCO. n = 7 and 8 cells for eLACCO1.1 and deLACCO, respectively (mean ± s.e.m.). d Schematic representation of l-lactate metabolism in the presence of 5.6 mM of extracellular glucose. e Representative fluorescence images of T98G cells expressing eLACCO1.1 or deLACCO treated with 5.6 mM glucose. Scale bars, 20 μm. f ΔF/F versus time for T98G cells expressing eLACCO1.1 or deLACCO. n = 8 and 8 cells for eLACCO1.1 and deLACCO, respectively (mean ± s.e.m.). Source data of c, f are provided as a Source Data file.
