Table 1 One- and two-photon photophysical parameters of eLACCO1.1.
From: A genetically encoded fluorescent biosensor for extracellular l-lactate
eLACCO1.1 | EGFP | ||
---|---|---|---|
l-lactate free | l-lactate bound | ||
Relative fraction of neutral chromophore (ρN) | 0.97 ± 0.07 | 0.57 ± 0.04 | 0.07a |
Relative fraction of anionic chromophore (ρA) | N/A | 0.43 ± 0.03 | 0.93a |
Neutral extinction coefficient (mM−1 cm−1, εN) | 34 | 38 | N/A |
Anionic extinction coefficient (mM−1 cm−1, εA) | 78 | 89 | 56.0b; 58.3c |
Neutral quantum yield (φN) | 0.20 ± 0.01 | 0.23 ± 0.01 | N/A |
Anionic quantum yield (φA) | 0.60 ± 0.03 | 0.78 ± 0.04 | 0.67b; 0.76c |
Molecular brightness (ρA × εA × φA) | N/A | 29.9 | 34.9b; 41.2c |
Neutral 1PA peak (nm) | 398 | 397 | N/A |
Anionic 1PA peak (nm) | 493 | 496 | 488b |
Two-photon brightness F2 (GM)d | 2.2 ± 0.3 (924 nm) | 16 ± 2 (924 nm) | 38 (911 nm)c |