Table 1 One- and two-photon photophysical parameters of eLACCO1.1.

From: A genetically encoded fluorescent biosensor for extracellular l-lactate

 

eLACCO1.1

EGFP

 

l-lactate free

l-lactate bound

 

Relative fraction of neutral chromophore (ρN)

0.97 ± 0.07

0.57 ± 0.04

0.07a

Relative fraction of anionic chromophore (ρA)

N/A

0.43 ± 0.03

0.93a

Neutral extinction coefficient (mM−1 cm−1, εN)

34

38

N/A

Anionic extinction coefficient (mM−1 cm−1, εA)

78

89

56.0b; 58.3c

Neutral quantum yield (φN)

0.20 ± 0.01

0.23 ± 0.01

N/A

Anionic quantum yield (φA)

0.60 ± 0.03

0.78 ± 0.04

0.67b; 0.76c

Molecular brightness (ρA × εA × φA)

N/A

29.9

34.9b; 41.2c

Neutral 1PA peak (nm)

398

397

N/A

Anionic 1PA peak (nm)

493

496

488b

Two-photon brightness F2 (GM)d

2.2 ± 0.3 (924 nm)

16 ± 2 (924 nm)

38 (911 nm)c

  1. Mean ± s.d. N/A, not applicable. Subscript A and N correspond to the anionic and neutral form of chromophore, respectively.
  2. aCalculated using the equation, log10([anionic]/[neutral]) = pH − pKa, where pH is 7.2 and pKa is 6.1 (ref. 16).
  3. bData from ref. 16.
  4. cData from ref. 17.
  5. dTwo-photon brightness is calculated as F2 = ρA × σ2,A × φA at the peak wavelength (in parentheses), where σ2,A is the peak two-photon absorption cross section.