Fig. 3: Confirmation of the incorporated hexose sugar as being mannose.
From: Synthesis and structure elucidation of the human tRNA nucleoside mannosyl-queuosine
a Workflow of the metabolic labelling study providing 13C6-galactose, 13C6-glucose, or 13C6-mannose in combination with an unlabelled carbon source (glucose or mannose) that suppresses metabolic interconversion of the carbohydrates and hence isotope scrambling; b Results of the LC-MS experiments showing quick incorporation of heavy sugar when feeding 13C6-mannose in presence of glucose, thereby confirming the identity of xQ as manQ; three independent experimental replicates were performed for each condition tested (coloured dots). Mean values are indicated by coloured horizontal lines, while whiskers enclose the 95%-confidence interval.
