Fig. 5: Chromatograms resulting from the co-injection of the synthetic manQ compounds 10, 11 and 4 with enzymatically digested total RNA from mouse liver analysed by UHPLC-MS/MS.
From: Synthesis and structure elucidation of the human tRNA nucleoside mannosyl-queuosine
a Co-injection of β-allyl-manQ 10 and α-homoallyl-manQ 11 show the appearance of a third peak, these compounds are therefore different from natural manQ; b Co-injection of α-allyl-manQ 4 with digested total RNA from mouse liver shows a complete signal overlap of 4 and natural manQ, thereby leading to an increased signal intensity of manQ (right) in comparison to the control sample without synthetic standard (left). This result shows that our synthetic compound 4 is identical to the naturally occurring manQ nucleoside and establishes its α-allyl-connectivity.
