Fig. 2: Deficiency of CRIg causes progression of ethanol-induced liver disease in mice.
From: CRIg on liver macrophages clears pathobionts and protects against alcoholic liver disease
Wild-type (WT), CRIg−/−, and CRIg−/−/Tlr2−/− mice were fed the Lieber DeCarli ethanol diet for 8 weeks. a Hepatic level of mRNA encoding CRIg in WT mice. b Serum levels of ALT. c Hepatic triglyceride content. d Representative oil red O-stained liver sections. e and f Hepatic levels of Il1b and Col1a1 mRNAs. g Representative sirius red-stained liver sections. h Quantification of the sirius red-stained liver sections. i and j Hepatic DNA levels of Firmicutes and Proteobacteria, normalized to total amount of bacteria using universal 16S primers. Scale bar = 100 μm. Results are expressed as mean ± s.e.m. (a–c, e, f, h–j). P values among groups of mice fed with control diet or ethanol diet are determined by two-sided Student t-test (a) or one-way ANOVA with Tukey´s post-hoc test (b, c, e, f, h–j). *P < 0.05, **P < 0.01, ***P < 0.001.
