Fig. 5: Single molecule identification of m6A sites.
From: RNA modifications detection by comparative Nanopore direct RNA sequencing
A Heatmap organised by hierarchical clustering showing the probability of A652, A1324, and A1535 in the β-actin gene to be modified in the WT and METTL3 KD samples. Each column corresponds to a single molecule. B Scatter plot with overlaid kernel density estimates showing the scaled median intensity vs the scaled log10 dwell time for each read covering A652, A1324 and A1535. Data points are colour coded according to the probability that the read belongs to the cluster of m6A modified reads. For visualisation purposes the x- and y-axis were limited to the +/-3 range. C Density plot showing the distribution of modification probability for A652, A1324, and A1535 of β-actin in WT (blue) and KD (red). D Bar chart showing the number of molecules identified in each of the 8 possible m6A configurations for the A652, A1324, and A1535 sites of β-actin. Each site was considered modified if the modification probability was >0.75. The shaded blue areas indicate the expected number of molecules in each given configuration under the null hypothesis of independence of the three modifications.
