Fig. 1: Efficient unnatural amino acids incorporation and generalizable synthetic auxotroph construction with the evolved chimeric Phe system. | Nature Communications

Fig. 1: Efficient unnatural amino acids incorporation and generalizable synthetic auxotroph construction with the evolved chimeric Phe system.

From: Directed-evolution of translation system for efficient unnatural amino acids incorporation and generalizable synthetic auxotroph construction

Fig. 1

Schematic illustration of the engineering of orthogonal translation systems with wild-type like efficiency in the presence of UAA and low background activity in the absence of UAA. The orthogonal translation system is used to construct UAA-dependent synthetic auxotroph by inserting amber codons into the essential gene. EP refers to essential protein.

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