Fig. 6: Application of UAA-dependent synthetic auxotroph in living mice. | Nature Communications

Fig. 6: Application of UAA-dependent synthetic auxotroph in living mice.

From: Directed-evolution of translation system for efficient unnatural amino acids incorporation and generalizable synthetic auxotroph construction

Fig. 6

a Bioluminescence intensity of UAA-dependent synthetic auxotroph constructed with 3TAGs in the dnaN gene in the presence or absence of AzF in vitro. b Bioluminescence imaging for UAA-dependent synthetic auxotroph in the absence (up) or presence (down) of AzF in living mice. Cells (∼1 × 108 cells/50 μl) were subcutaneously injected into the mice (left legs, DH10B cell; right legs, UAA-dependent synthetic auxotroph expressing fLuc protein). The bioluminescent signal was measured in whole animals at 24 h after injection. c Statistic analysis of bioluminescent value in b. Error bars represents ±standard error of the mean from three biologically independent experiments. Source data are provided as a Source Data file.

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