Fig. 5: 2A binds to the 70S ribosome via interactions with the 16S rRNA.
From: Structural and molecular basis for Cardiovirus 2A protein as a viral gene expression switch
a Cryo-EM analysis of a complex formed between initiated E. coli 70S ribosomes and EMCV 2A. Images (×75,000) were recorded on a Titan Krios microscope. Representative micrograph from dataset of 5730 images. b Cryo-EM map at 2.7Å resolution after focused classification and refinement. Three copies of 2A (orange, red, yellow) are bound to the 16S rRNA of the small (30S) subunit (blue ribbon). c Close-up view of the 2A-binding site. Ribbon diagrams of 2A (coloured as above) and ribosomal RNA (purple) are shown. Protein N- and C- termini are labelled. d Superposition of the three copies of 2A reveals a common RNA-binding surface with conformational flexibility. Residues involved in rRNA binding are labelled and shown as sticks.
