Fig. 4: Fate mapping of Hhip-CreERT2-expressing suture mesenchyme.
From: Single-cell analysis identifies a key role for Hhip in murine coronal suture development
a Graph abstraction of the relationships between CS8-1 to CS8-9. Inset shows coloring by cluster identity. The root state of the inferred trajectory is indicated by the number 1 at the center of the cells with the earliest pseudotime. b TdTomato-expressing (tdTomato + ) cells at postnatal day (P)2 after 1 day of induction. n = 3. c TdTomato+ cells at P3 after two consecutive days of induction. n = 3. d TdTomato+ cells at P6 after five consecutive days of induction. n = 1. e TdTomato+ cells at P30 after 1 day of induction at P1. Dashed box is enlarged at right to show cluster of tdTomato+ osteoblasts within ALPL domain (dashed lines). n = 1. f TdTomato+ cells at P90 after two consecutive days of induction at P1 and P2. n = 3. g TdTomato+ cells at P90 after five consecutive days of induction between P1-P5. n = 2. White and yellow empty/solid arrowheads indicate tdTomato+ osteoblasts/osteocytes in parietal and frontal bones, respectively. f frontal bone, p parietal bone. Sections are in the sagittal plane. Scale bars, 100 μm except inset in e, 50 μm.
