Fig. 6: Coronal suture dysgenesis in Hhip−/− mice at E18.5.
From: Single-cell analysis identifies a key role for Hhip in murine coronal suture development
a Staining for alkaline phosphatase activity (ALPL, red) in preosteoblasts and osteoblasts for WT and Hhip−/− coronal sutures. f, frontal bone; p, parietal bone; sm, suture mesenchyme. b Immunohistochemistry for RUNX2 (green). Sections are the same as shown in a. c Immunohistochemistry for SP7 (green). d Proliferation detected by EdU incorporation (green). Sections are counterstained for ALPL activity (red) and DAPI (blue). e Quantification of EdU incorporation in osteogenic fronts (OF) and suture mesenchyme (SM). WT and Hhip−/− are indicated in black and red, respectively. Asterisk indicates a significant difference using the two-tailed Student’s t-test (P = 0.030). n = 6 WT and six Hhip−/−. f Quantification of cell numbers per section in OFs and SM. Asterisk indicates a significant difference using the two-tailed Student’s t-test (P = 0.018, 0.008, and 0.00004, respectively). n = 6 WT and six Hhip−/−. g TUNEL assay for apoptotic cells. Inset, example of an apoptotic cell in non-sutural tissue on the same slide. White dashed outlines, frontal and parietal bones. Data in e and f are presented as dot plots showing mean ± standard deviation. Results presented in a, b, c, and g are representative of n = 3 WT and three Hhip−/− independent samples. Sections are in the transverse plane. Scale bar, 100 μm. Source data are provided as a Source Data file.
