Table 3 Mean fluorescence intensity I′ detected per cell after t = 48 h incubation at C′CNDs = 400 μg mL−1, corresponding to the last time point in Fig. 3.

From: Influence of the chirality of carbon nanodots on their interaction with proteins and cells

 

Serum-supplemented medium

Serum-free medium

 

HeLa

THP-1

HeLa

THP-1

 

Flow cytometry

Flow cytometry

Confocal microscopy

Flow cytometry

Flow cytometry

Confocal microscopy

I′(R-CNDs) (a.u.)

798

480

30.2

971

492

46.4

I′(S-CNDs) (a.u.)

907

601

61.3

1065

654

112.5

I′(N-CNDs) (a.u.)

381

455

14.2

378

149

21.5

〈I′〉

695

512

35.2

805

432

60.1

ΔI′(R-CNDs) 〈I′〉–1

0.15

−0.06

−0.14

0.21

0.14

−0.23

ΔI′(S-CNDs) 〈I′〉–1

0.30

0.17

0.74

0.32

0.52

0.87

ΔI′(N-CNDs) 〈I′〉–1

−0.45

−0.11

−0.60

−0.53

−0.65

−0.64

ΔI′(S-CNDs) 〈I′〉–1 – ΔI′(R-CNDs) 〈I′〉–1

0.16

0.24

0.88

0.12

0.38

1.10

  1. The full tables are provided in the Supplementary Information (Supplementary Tables 6–8). For all three types of CNDs the mean value for each incubation condition is given as 〈I′〉 = (I′(R-CNDs) + I′(S-CNDs) + I′(N-CNDs)) 3–1. Deviations are calculated as ΔI′(j) = (I′(j) − 〈I′〉)〈I′〉–1 (j = R-CND, S-CND, N-CND). ΔI′〈I′〉–1 = ΔI′(S-CNDs) 〈I′〉–1 − ΔI′(R-CNDs) 〈I′〉–1 tells the relative difference in fluorescence intensity per cell between S-CNDs and R-CNDs.
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