Fig. 1: Time-resolved cryo-EM of translocation with EF-G and GTP.
From: Time-resolved cryo-EM visualizes ribosomal translocation with EF-G and GTP
a Scheme of the translocation reaction of the 70S•mRNA•fMet-tRNAfMet•Pro-tRNAPro complex with EF-G•GTP. b Segmented cryo-EM maps of 8 states of the translocation reaction, and their assignment as substrates, EF-G-bound intermediates, or products of the reaction. The maps are colored to show the 50S ribosomal subunit (light blue), 30S ribosomal subunit body (yellow) and head (tan), tRNAfMet (dark blue), tRNAPro (green), mRNA (magenta) and EF-G (red). c Relative abundance of substrates (blue), EF-G intermediates (red), and translocation products (green) over time, obtained from particle distributions in cryo-EM datasets. d Domain organization of EF-G; Arabic numerals denote the five conserved domains of the elongation factor. e Cryo-EM density of the EF-G GTPase center in the transient pre-translocation and pre-Pi-release state (III). For additional density views, see Fig. 4 and Supplementary Figs. 3, 4, and 5.
