Fig. 3: Positions and interactions of EF-G in translocation intermediates.
From: Time-resolved cryo-EM visualizes ribosomal translocation with EF-G and GTP
a–b Positions of EF-G and tRNAs relative to the decoding center (yellow) in Structures III and IV. c Superposition of EF-G in Structures III and IV demonstrates an overall similar extended conformation. d Movement of EF-G relative to the 30S subunit from Structure III (gray) to IV (colored). Structures are aligned on 16S rRNA and colored ribosomal elements are from Structure III. e Movement of EF-G relative to the 30S subunit from Structure IV (colored) to V (blue-gray). Structures are aligned on 16S rRNA and colored ribosomal elements are from Structure IV. f Buried surface area (contact area) showing the extent of interactions of EF-G domains with the ribosome, mRNA, and/or tRNA in Structures III to V.
