Table 1 Proteomic analysis of amyloid deposits in human TTRS52P transgenic mice.

From: Plasmin activity promotes amyloid deposition in a transgenic model of human transthyretin amyloidosis

 

Mouse (line)

Tissue

Human TTRS52P

Amyloid-signature proteins Mascot score (Matches/USP)

Mascot score (Matches/USP)

Coverage (%)

ApoA4

ApoE

SAP

Vitronectin

Seeded, amyloidotic

1.14 (O5)

Tongue

341 (10/7)

85

27 (2/2)

170 (6/6)

–

103 (5/4)

2.10 (O5)

Tongue

459 (13/8)

75

33 (1/1)

91 (3/3)

–

63 (3/3)

14.4 (N4)

Heart

1793 (60/8)

79

148 (7/5)

22 (1/1)

39 (1/1)

54 (3/2)

14.4 (N4)

Tongue

1431 (56/8)

79

170 (6/5)

26 (1/1)

–

53 (2/2)

12.9 (N4)

Tongue

6354 (185/15)

100

782 (24/16)

571 (23/12)

201 (6/5)

250 (11/7)

12.15 (N4)

Tongue

6324 (184/14)

96

904 (28/17)

699 (28/12)

236 (8/6)

404 (15/6)

12.22 (N4)

Tongue

4572 (132/14)

95

647 (24/16)

465 (20/11)

232 (8/6)

224 (10/5)

Unseeded non-amyloidotic controls

164894 (N4)

Tongue

81 (3/2)

20

–

–

–

–

22.13 (N4)

Tongue

40 (1/1)

10

–

–

–

–

22.15 (N4)

Tongue

32 (2/2)

28

–

–

–

–

  1. LC-MS/MS analysis of laser capture microdissected amyloid (seeded, amyloidotic) or equivalent control samples (unseeded, non-amyloidotic). Mascot analysis identifies human TTR in the microdissected material; the 52P-containing peptides 49–70 and 49–76 were present in most samples. The Mascot scores are consistent with the relative amounts of amyloid in the tissues (small in seeded line O5 mice, large in seeded line N4 mice and none in unseeded line N4 mice). Our normal minimum acceptance score for clinical amyloid proteins96 is 80, and for signature proteins it is 20. Human TTR was detected in the control samples, probably reflecting the high concentration of TTR in the circulation of line N4 mice, but the scores were very low. Identification of multiple amyloid-associated proteins in the seeded, but not unseeded control mouse samples is consistent with the amyloid content of the deposits.
  2. USP unique significant peptide, ApoA4 apolipoprotein A4, ApoE apolipoprotein E, SAP serum amyloid P-component.
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