Fig. 2: Biomimetic phospholipid- and glycolipid-derived nanoparticles for mRNA delivery. | Nature Communications

Fig. 2: Biomimetic phospholipid- and glycolipid-derived nanoparticles for mRNA delivery.

From: Biomimetic nanoparticles deliver mRNAs encoding costimulatory receptors and enhance T cell mediated cancer immunotherapy

Fig. 2

a Luminescence intensity of phospholipid- (PL) and glycolipid-derived (GL) nanoparticles delivering firefly luciferase (Fluc) mRNA to E.G7 cells. b Cryo-TEM image of PL1-mRNA nanoparticles (a representative image from one of five independent fields of view in a single experiment). Scale bar = 50 nm. c PL1 nanoparticles delivered GFP mRNA to E.G7 cells. d PL1-CD137 induced CD137 expression in E.G7 cells. e PL1-OX40 induced OX40 expression in EG.7 cells. f Scheme of GFP expression in B16F10 tumors after a single injection of free GFP mRNA or PL1-GFP. g GFP expression in CD4+, CD8+ T cells, after a single intratumoral injection with GFP mRNA (n = 4) or PL1-GFP mRNA (n = 5) in B16F10 tumors. Data in c, d and e are representative of three independent experiments. All data are presented as the mean ± S.E.M. Statistical significance in c, d, e was analyzed using one-way ANOVA followed by Dunnett’s multiple comparison test, and data in g was analyzed using the two-tailed Student’s t-test. *P < 0.05; ***P < 0.001; ****P < 0.0001. Source data are provided as a Source Data file.

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