Fig. 5: LIFR and SHP1 positively regulate ferroptosis while LCN2 negatively regulates ferroptosis.
From: A targetable LIFR−NF-κB−LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis
a-d LIFR-knockdown (a, b) or SHP1-knockdown (c, d) HT1080 cells were treated with 10 μM erastin for 0, 5, or 10 h. a, c: staining of 7-aminoactinomycin (7-AAD) and annexin V. b, d: the percentage of annexin V and 7-AAD double-negative population. e The percentage of annexin V and 7-AAD double-negative population in LIFR-knockdown HT1080 cells treated with 0.5 μM RSL3 for 12 h, 50 μM FIN56 for 6 h, or 10 μM FINO2 for 24 h. Supplementary Figure 6e shows representative flow cytometry plots. f The percentage of annexin V and 7-AAD double-negative population in SHP1-knockdown HT1080 cells treated with 0.5 μM RSL3 for 12 h, 50 μM FIN56 for 6 h, or 10 μM FINO2 for 24 h. Supplementary Figure 6f shows representative flow cytometry plots. g The percentage of annexin V and 7-AAD double-negative population in LCN2-knockdown HT1080 cells treated with 10 μM erastin for 0, 4, or 8 h, alone or in combination with liproxstatin-1 (lip-1, 10 μM) or DFO (100 μM). Supplementary Figure 7b shows representative flow cytometry plots. h The percentage of annexin V and 7-AAD double-negative population in LCN2-knockdown HT1080 cells treated with 0.5 μM RSL3 for 10 h, 50 μM FIN56 for 3 h, or 10 μM FINO2 for 12 h, alone or in combination with liproxstatin-1 (lip-1, 10 μM) or DFO (100 μM). Supplementary Fig. 7c shows representative flow cytometry plots. i, j Lipid peroxidation levels in LIFR-knockdown (i) and SHP1-knockdown (j) HT1080 cells treated with 10 μM erastin for 3 h or 0.5 μM RSL3 for 4 h. k Lipid peroxidation levels in LCN2-knockdown HT1080 cells treated with 10 μM erastin for 3 h or 0.5 μM RSL3 for 4 h, alone or in combination with liproxstatin-1 (10 μM) or DFO (100 μM). Lipid peroxidation levels were gauged by C11-BODIPY staining in i–k. Statistical significance in b and d–k was determined by a two-tailed unpaired t-test. Error bars are s.e.m. n = 3 samples per group. Source data are provided as a Source Data file.
