Fig. 1: Asthma induction inhibits Nf1OPG formation.
From: Asthma reduces glioma formation by T cell decorin-mediated inhibition of microglia
a Schematic representation of the treatments used to induce asthma in Nf1OPG mice. Nf1OPG mice were treated between 4 and 6 weeks of age with either OVA or HDM, while control Nf1OPG mice received PBS only (vehicle). Isolated optic nerves were analyzed at 12 weeks of age. OVA and HDM treatments reduced (b) optic nerve volumes (WT, n = 6; PBS, n = 14; OVA, n = 6; HDM, n = 8) and (c) percentage of proliferating (%Ki67+) tumor cells within the optic nerves of Nf1OPG mice relative to the vehicle-treated groups (WT, n = 6; PBS, n = 8; OVA, n = 8; HDM, n = 9). No difference in microglia (%Iba1+ cells) or T cell (CD3+ cells) content were observed between OVA- or HDM-treated mice and their respective controls. Reduced optic nerve volumes (e) and proliferation (%Ki67+ cells; f) were also observed at 24 weeks of age after OVA and HDM treatment (d) between 4 and 6 weeks of age (WT, n = 6; PBS, n = 13; OVA, n = 8; HDM, n = 7). Data are presented as the means ± SEM. c, f Scale bars, 40 µm. One-way ANOVA with Bonferroni post hoc correction. Exact P values are indicated within each panel (ns, not significant). From left to right in each panel: b P < 0.0001, P < 0.0001, P < 0.0003; c P = 0.0001, ns; e P < 0.0001, P = 0.0040, P = 0.0008; f P < 0.0001, P < 0.0001, P < 0.0001, ns, P < 0.0001, ns.
