Fig. 2: Asthma reduces Nf1^OPG Ccl5, but not Ccl4, levels.

a Schematic representation of the journey of T cells from the periphery (blood) into the optic nerve and draining through the cervical lymph node. b Increased Ccl4 mRNA expression was observed in optic nerves (n = 4) of Nf1^OPG mice relative to blood (n = 5) and draining cervical lymph nodes (n = 5). One-way ANOVA with Bonferroni post hoc correction. c Schematic representation of the interactions between T cells and microglia in the optic nerve and draining cervical lymph node. d No change in Ccl4 mRNA expression was found in the optic nerves of Nf1^OPG mice treated with OVA (n = 4) or HDM (n = 3) relative to vehicle-treated (n = 7) mice. One-way ANOVA with Bonferroni post hoc correction. e Decreased optic nerve Ccl5 mRNA expression was observed in both OVA- (n = 4) and HDM- (n = 3) treated mice relative to PBS-treated controls (n = 7). One-way ANOVA with Bonferroni post hoc correction. f While Ccl4 gene expression was increased in the cervical lymph nodes of Nf1^OPG mice relative to controls (WT, n = 3; Nf1^OPG, n = 3), Two-tailed Student’s t test. (g) No change in cervical lymph node Ccl4 mRNA expression was observed between OVA- or HDM-treated mice (OVA, n = 4; HDM, n = 3) relative to controls (n = 7). One-way ANOVA with Bonferroni post hoc correction. Data are presented as the means ± SEM. Exact P values are indicated within each panel (ns, not significant). From left to right in each panel: b P < 0.0001, P = 0.0460; d ns, ns; e P = 0.0069, P = 0.0359; f P = 0.0058; g ns.