Fig. 4: Asthma-induced T cell decorin inhibits microglia Ccl5 level.

a Heatmap showing differential gene expression in CD3⁺ T cells from PBS- versus OVA-treated Nf1^OPG mice (n = 5). b Top transcripts increased in T cells from OVA-, relative to PBS-treated, Nf1^OPG mice calculated with DESeq2 were filtered for only those genes with P values ≤ 0.01, false discovery rates ≤ 0.05, and log fold changes greater or equal to ±5. P values, false discovery rate (FDR), and fold changes are shown in a tabular form. c Increased Dcn gene expression was detected in both splenic CD4⁺ and CD8⁺ T cells from OVA-treated Nf1^OPG mice relative to PBS-treated controls. Data are presented as the means ± SEM of n = 3 independent biological samples. d DCN RNA expression was increased in CD4⁺ and CD8⁺ T cells from patients with non-steroid-dependent asthma (n = 4) relative to healthy controls (n = 6; GSE31773). Two-tailed Student’s t test. Exact p values are indicated within each panel. e Similar levels of Dcn mRNA expression were observed in T cells from the blood (n = 5), optic nerves (n = 5), and cervical lymph nodes (n = 5) in Nf1^OPG mice treated with OVA. Data are presented as the means ± SEM of n = 5 independent biological samples. One-way ANOVA with Bonferroni post hoc correction. f Increased Dcn levels were detected in CD3⁺ T cell-conditioned medium (TCM) from OVA-treated Nf1^OPG mice relative to PBS-treated controls by ELISA. Two-tailed Student’s t test. Exact P values are indicated within the panel. Data are presented as the means ± SEM of n = 3 independent biological samples. From left to right in each panel: c P = 0.0006, P = 0.0002; d P = 0.0007, P = 0.0032; e ns; f P = 0.0003.