Fig. 1: Cryo-EM images and analysis of ATTR fibrils from the vitreous body.
From: Structural basis for transthyretin amyloid formation in vitreous body of the eye
a Representative cryo-EM micrographs: single protofilament, black arrowhead; twisted-dimer, gray arrowhead; twisted-trimer, white arrowhead. The 2D class averages of each filament type are shown as insets. Scale bars, 50 nm. b Estimated crossover distances and maximum widths of ATTR fibrils in the micrographs. Box plots show the mean values ± standard deviations from n = 25 independent measurements for each type of fibril. The cross-distances are 147.7 ± 8.5 nm for monomer, 148.9 ± 13.4 nm for twisted-dimer, and 163.3 ± 13.1 nm for twisted-trimer. The maximum width for monomer is 7.5 ± 0.5 nm, for twisted-dimer is 14.9 ± 0.9 nm and 21.3 ± 0.8 nm for twisted-trimer. The maximum width and helical periodicity were analyzed by Fiji (ImageJ) 1.52 and GraphPad Prism 9.2.0 softwares. Source data for (b) are provided in a Source data file.
