Fig. 5: QD-Cat-RGD-based RT for promoting DC maturation and antigen presentation in TDLNs and consequently inducing T cell-driven antitumor immunity.

a Representative flow cytometric analysis images and relative quantification of co-stimulatory molecules CD80⁺CD86⁺ gating on CD45⁺ CD11c⁺ cells in TDLNs. b Representative flow cytometric analysis images and relative quantification of MHC-II expression on CD11c⁺CD45⁺ cells in the TDLNs. c Representative flow cytometric analysis images and relative quantification of CD3⁺ T cells gating on CD45⁺ in tumors. d Representative immunofluorescence images of tumors showing CD8⁺ T cells and relative quantification of CD8⁺CD3⁺ T cells gating on CD45⁺ cells in tumors by flow cytometry. Scale bars = 50 μm. The immunofluorescent images were representative of those generated from three mice each group. e Representative flow cytometric analysis images and relative quantification of CD44⁺CD62L⁺ Tcm cells gating on CD8⁺CD3⁺CD45⁺ cells in the TDLNs. All data are presented as the mean ± s.e.m. (n = 5 per group) and n represents the number of independent animals. Statistical significance was calculated via one-way ANOVA with Tukey’s multiple comparisons test. FMO fluorescence-minus-one. TDLN tumor-draining lymph node. Source data are provided as a Source Data file.