Fig. 4: Mutagenesis of the gene, PxCad, mediated by the CRISPR/Cas9 genome editing system for diamondback moth.
From: Large-scale genome-wide study reveals climate adaptive variability in a cosmopolitan pest
a Representative sequencing trace of the PCR fragment from mutated G0 adults with multi-peaks at the cleavage site and representative sequence of the diverse indel mutations flanking the sgRNA target sites of PxCad in the G0 individuals. The Δ46 in red denotes the deletion mutation kept establishing the G88-Cad mutant (MU) strain. b SDS-PAGE profile of BBMV protein from the WT (G88) strain and the MU (G88-Cad) strain. c Details of the 19 peptides specific to PxCad identified from the WT (G88) strain. d Map of the full-length PxCad protein showing the position of 19 peptides (red arrows) specific to PxCad identified from the WT (G88) strain and absent from the MU (G88-Cad) strain. Numbers indicate the position of amino acid residues.
