Fig. 1: Schematic representation of the main sample preparation steps and follow-up analyses of the CAMPI study. | Nature Communications

Fig. 1: Schematic representation of the main sample preparation steps and follow-up analyses of the CAMPI study.

From: Critical Assessment of MetaProteome Investigation (CAMPI): a multi-laboratory comparison of established workflows

Fig. 1

The figure consists of three parts: (i) Pre-symposium work by the organizers (left panel). The two samples (SIHUMIx and fecal sample) were, prior to the symposium, aliquoted and distributed over the participating laboratories. (ii) Pre-symposium work by participants (middle panels). Every used method by the participants, going from cell disruption to mass detection, is displayed. (iii) Post-symposium work by participants (right panel). The bioinformatics analyses, i.e., database creation and database search for peptide and protein identification, were harmonized to make the results between all participating laboratories comparable. The stock icons in the leftmost column were obtained from vecteezy.com, flaticon.com, and labicons.net.

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