Fig. 5: Plastid ROS influences chlorophyll content and PRGs expression in a Fe-dependent manner.
From: Interdependent iron and phosphorus availability controls photosynthesis through retrograde signaling
a Relative mRNA abundance of bZIP58 in shoots of plants expressing JUNGBRUNNEN1p::bZIP58 in the bzip58 (Line A1) and bzip58xpht4;4 (Line B1) mutant backgrounds. Plants were grown in the presence of iron and phosphorus (+Fe+P) for 7 days and transferred to +Fe+P, −Fe+P, or −Fe+P+AsA for 76 h. Data shown are the means from five biologically independent samples. Error bars indicate 95% confidence intervals. Asterisks indicate statistically different bZIP58 expression in the treatment compared to the control (+Fe+P) (P value < 0.01, two-sided Student’s t test). b Representative images of wild-type Col-0, Line A1 and Line B1 grown for 1 week under +Fe+P and transferred to three different media conditions (+Fe+P, −Fe+P, or −Fe+P+AsA) for 2 additional weeks. Scale bars: 7 mm. The experiment was repeated with similar results three times. c Total chlorophyll content in Col-0, Line A1 and Line B1 grown under +Fe+P, −Fe+P, or −Fe+P+AsA. FW: fresh weight. Data shown are from ten plants representing three independent experiments. Center lines show the medians; box limits indicate the 25th and 75th percentiles; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles. d Relative mRNA abundance of PRGs genes (PSBTN (AT3G21055), AT1G5140, and PTAC16 (AT3G46780)) in shoots of Col-0, bzip58, Line A1, bzip58 pht4;4, and Line B1. Plants were grown under +Fe+P for 7 days and transferred to +Fe+P, −Fe+P, or −Fe+P+AsA for 76 h. Data shown are the means from five biologically independent samples. Error bars indicate 95% confidence intervals. For c, d, letters above bars or boxes represent statistically different means at P < 0.05 (one-way ANOVA with a Duncan post hoc test).
