Fig. 5: EpoR regulates cell size independently of HRI.

a Epor^−/− and doubly deleted Epor^−/−Hri^−/− E12.5 embryos with wild-type littermates. b Cell and nuclear diameters in fetal livers from either Epor^−/− or Epor^−/−Hri^−/− embryos, transduced with either EpoR or Bcl-x_L, at t = 48 h post transduction. Individual sample contour plots are overlaid on scatter plots (each dot is one cell). Red dots indicate distributions’ medians. c Summary data for cell and nuclear area, for two independent experiments as in (b), each containing all 4 genotype/retrovirus combinations. Data are mean ± SD for each cell population. Each transduced population consisted of pooled fetal liver cells from either Epor^−/− or Epor^−/−Hri^−/− embryos. Cell diameter data for all genotypes is significantly different (p = 0.0008, one-way ANOVA between population means); On the Hri^−/− Epor^−/− background, cell diameter is significantly different between Bcl-x_L and EpoR-transduced cells (p = 0.019, one-way ANOVA from two independent experiments). d Imaging flow cytometry of representative Lin⁻hCD4⁺Ter119⁺ erythroblasts from each of the genotype/retroviral combinations at t = 48 h. Representative of at least 3 independent experiments. Scale bar = 10 µm. e Epor^−/− and Epor^−/−Hri^−/− reticulocyte cell diameter, from cultures transduced with either EpoR or Bcl-x_L. Representative of 2 experiments. Violin lines mark the 25th, 50th, and 75th percentile with a white circle marking the mean.