Fig. 3: hRpn13 β-hairpin shifts to bury XL5 benzoic acid.

Ribbon diagram structures of hRpn13 Pru ligated to XL5 (colored as in Fig. 2g) to highlight key interactions, which are indicated by gray lines with distances (Å) included. a Comparison of XL5-ligated and free hRpn13 Pru (PDB 5IRS, green) structures with an expansion (dashed rectangles) in the right panel and hRpn13 W108 included. b, c Structural comparison of XL5-ligated hRpn13 (colored as Fig. 2g) and hRpn2-bound hRpn13 (PDB: 6CO4) with hRpn2 colored as in Fig. 1c. d Expanded view highlighting hRpn13 M31, L33, V38, and V93 interaction with the XL5 benzoic acid group. e Expanded view of XL5 4-methyl benzamide interaction with hRpn13 V38, T39, and P40.