Fig. 4: Synaptic gene expression and YY1 regulatory activity are decreased in CUS L2/3 excitatory neurons. | Nature Communications

Fig. 4: Synaptic gene expression and YY1 regulatory activity are decreased in CUS L2/3 excitatory neurons.

From: Neuronal Yin Yang1 in the prefrontal cortex regulates transcriptional and behavioral responses to chronic stress in mice

Fig. 4

a L2/3 DEGs are enriched for synapse- and axonal-related gene ontology (GO) terms (FDR < 0.5). b Upregulated L2/3 DEGs (pink) are enriched for ribosomal-related GO terms. Downregulated L2/3 DEGs (blue) primarily encode synapse-related proteins. c Heatmap of CUS L2/3 SCENIC results showing 26 transcription factors that are significantly upregulated in activity and 15 that are downregulated in activity relative to controls. Legend depicts normalized mean AUC values for CUS nuclei relative to control nuclei. d Percentage of L2/3 control and CUS nuclei expressing Yy1 (Unpaired t-test, P = 0.017; n = 4 per group). e YY1 target genes in L2/3 neurons encode nuclear proteins and RNA Pol II-mediated transcription-related GO terms. f Representative RNAscope images for Yy1 and Cdkl5 in medial PFC tissues taken from control and CUS mice. g Quantification of Yy1 and Cdkl5 mRNA puncta number per cell in layer 2/3 and layer 6 are shown on the right (Linear mixed-effect model; P = 0.02; n = 30 cells per animal, n = 3 animals per treatment). *P < 0.05. Error bars represent s.e.m. Source data are provided as a Source data file.

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