Fig. 2: Subcellular distribution of lysine-succinylation proteins in human brains.

a Subcellular distribution of succinylated-K proteins identified by Cytoscape and stringAPP software. The majority of succinylated-K proteins are mitochondrial. b Overlap of succinylated-K proteins located in the mitochondrion, nucleus, cytosol, and plasma membrane. The details of the subcellular distribution of individual proteins are shown in Supplementary Data 2. c The extent of succinylation of individual proteins and their enrichment in mitochondria. Distribution of the number of succinylation sites per protein in all of the succinylated proteins (purple bars) or succinylated mitochondrial proteins (green bars) as classified by Cytoscape and stringAPP. d The succinylation sites were analyzed for seven amino acids up- and downstream of the lysine residue using Motif-X. The height of each letter corresponds to the frequency of that amino-acid residue in that position. The central blue K refers to the succinylated lysine. e Heatmap of the 15 amino-acid compositions of the succinylated site showing the frequency of the different amino acids in specific positions flanking the succinylated lysine. The different colors of blocks represent the preference of each residue in the position of a 15 amino-acid-long sequence context (green indicates greater possibility, while red refers to less possibility). Source data are provided as a Source Data file.