Fig. 6: The cellular heterogeneity of myeloid compartment in ESCC.

A Violin plots showing expression of representative markers in either DC (dendritic cell) or (B) monocyte and macrophage subsets. C Dotplot showing the expression of top 10 differentially expressed genes across monocyte and macrophage subsets. D Scatter plot showing the correlation of M1 and M2 gene signatures in individual macrophage subsets using all myeloid cells as background. E The expression ratio of M2 over M1 gene signatures. F Histogram of the distribution of myeloid cells along the Diffusion-1 component from either the PBMC (peripheral blood mononuclear cells), tumor or nonmalignant samples. G Diffusion component analysis of the myeloid compartment displayed in 3D plot showing the first 3 diffusion components colored by either sample origin or (H) annotated subsets. The 1st diffusion component reflected a trajectory from blood monocytes to tissue monocytes/macrophages, the 2nd diffusion component reflected the activation of monocyte-derived macrophages in tissue, and the 3rd diffusion component reflected a trajectory from pDCs (plasmacytoids dendritic cell) to more mature DC subsets. I–K 3D plots showing the correlation of representative genes with each diffusion component. Source data are provided as a Source Data file.