Fig. 7: Constitutive activation of β-CATENIN leads to a dysregulation of the ChP transcriptome.
From: Constitutive activation of canonical Wnt signaling disrupts choroid plexus epithelial fate
(a) A cartoon depicting the microdissection E14.5 ChP and hippocampus for RNAseq analysis. (b) Principal Component Analysis (PCA) of the RNAseq datasets. (c, d) Choroid plexus-enriched genes are downregulated, and hippocampus/neuron-enriched genes are upregulated in the Lmx1aCre::β-catenin GOF ChP. In (c) log2 fold changes of 31 representative choroid plexus-enriched genes and 20 neuron-enriched genes are plotted (Padj < 0.05), (d) heatmap and hierarchical clustering show read counts of 500 choroid enriched and 500 hippocampus enriched genes from the RNA-seq dataset (Supplementary data 1). (e, f) Venn diagram showing the overlap between genes corresponding to the ChP secretome (from Lun et al. 2015.) and differentially expressed genes in the Lmx1aCre::β-catenin GOF versus control ChP and heat map of selected genes plotted as read counts (e) shows the dysregulated genes belong to various known pathways of cell-cell signaling (f). Color bars in (d, f): blue (low expression), red (high expression). For RNAseq experiments (c, d, and f) N = 2 biologically independent replicates. Further information on replicates and reproducibility for this figure is mentioned in the “Statistics and Reproducibility” section of the Methods and Supplementary data 1. Source data are provided as a Source Data file.
