Fig. 8: Cell-type classification of genes dysregulated upon constitutive activation of β-CATENIN.
From: Constitutive activation of canonical Wnt signaling disrupts choroid plexus epithelial fate
(a) comparison of RNA-seq data from E14.5 Lmx1aCre::β-catenin GOF ChP with scRNA-seq data from E16.5 ChP (Dani et al., 2021). Genes that were differentially expressed (DE) between the Lmx1aCre::β-catenin GOF ChP and control ChP (Supplementary data 1) were compared with the genes enriched in Epithelial/Neuronal/Endothelial/Mesenchymal/Glial/Immune cell groups identified in Dani et al., 2021. For each cell group, the DE list contained genes that were either upregulated (blue), downregulated (orange), or unchanged/undetected (grey). (b, c) Log2 fold change (Padj < 0.05) selected choroid plexus epithelial genes (74 downregulated) and neuronal-enriched genes (122 upregulated and 21 downregulated) that were dysregulated in the Lmx1aCre::β-catenin GOF ChP upon comparison with Dani et al., 2021. Bar graphs are color coded as Upregulated (blue), downregulated (orange). For RNAseq experiments (a, b, and c) N = 2 biologically independent replicates. Further information on replicates and reproducibility for this figure is mentioned in the “Statistics and Reproducibility” section of the Methods and Supplementary data 1. Source data are provided as a Source Data file.
