Fig. 9: Differentiation of hESC organoid cultures using a ChPe differentiation-promoting protocol.
From: Constitutive activation of canonical Wnt signaling disrupts choroid plexus epithelial fate
a Schematic presentation describing the organoid culture protocol. (b) Bright-field images of organoids at different stages of development. Representative images from N = 48 organoids (biologically independent replicates) examined over 3 separate experiments. (c, d) Immunostaining reveals the presence of choroid markers OTX2 and TTR in the perimeter of the protrusions of a day 40 organoid. Boxed region (c, d) is shown at high magnification in the adjacent panels. (e) AQP1 immunostaining in Day 40 organoids is similar to that seen in the E14.5 mouse ChP. (c–e) Representative images of sections taken from N = 12 organoids (biologically independent replicates) examined in 2 separate experiments. (f) Heatmap and hierarchical clustering generated from normalized reads obtained by MARS-Seq show upregulation of ChP-enriched genes by day 30 of the organoid culture (N = 3 biologically independent replicates). Scale bars: 200 μm (all panels in b); 100 μm (c); 10 μm (all panels in d and e). Source data are provided as a Source Data file.
