Fig. 2: EZH2 deficiency decreases MYCN and MYC expression and transcriptional activity in neuroblastoma cells. | Nature Communications

Fig. 2: EZH2 deficiency decreases MYCN and MYC expression and transcriptional activity in neuroblastoma cells.

From: EZH2 depletion potentiates MYC degradation inhibiting neuroblastoma and small cell carcinoma tumor formation

Fig. 2

a Immunoblot to detect MYCN or MYC levels upon EZH2 depletion with specific sgRNAs in MYCN-amplified (Kelly, SK-N-BE2 and SMS-KAN) and MYCN-nonamplified (SK-N-AS, SH-SY5Y and NBLS) neuroblastoma cells. Actin was used as a loading control. The experiments were independently repeated three times with similar results. b Heatmap presentation of genome-wide MYCN and pol II occupancies in MYCN-amplifed SK-N-BE2 cells analyzed by ChIP-Seq. c Genome browser views of MYCN occupancy in the NCL and PRMT5 loci in SK-N-BE2 cells with or without EZH2 depletion. d Binding of MYCN to the NCL or PRMT5 promoter was analyzed by ChIP-qPCR in SK-N-BE2 cells with or without EZH2 depletion. Averages of fold enrichment between anti-MYCN and the isotype IgG control are shown. Graph shows mean ± SD from triplicates; significance was determined by two-way ANOVA test followed by Tukey’s correction. e Real-time qPCR analysis of representative MYCN target genes in SK-N-BE2 cells upon EZH2 depletion. Graph shows mean ± SD from triplicates; significance was determined by unpaired two-tailed Student’s t-test. Source data are provided as a Source data file.

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