Fig. 7: Depletion of EZH2 reduces MYC(N) expression and suppresses growth of MYC(N)-driven small cell carcinoma cells and MYC-driven Burkitt’s lymphoma cells. | Nature Communications

Fig. 7: Depletion of EZH2 reduces MYC(N) expression and suppresses growth of MYC(N)-driven small cell carcinoma cells and MYC-driven Burkitt’s lymphoma cells.

From: EZH2 depletion potentiates MYC degradation inhibiting neuroblastoma and small cell carcinoma tumor formation

Fig. 7

a–c Analysis of MYCN or MYC expression and cell proliferation upon EZH2 depletion by specific sgRNAs or administration of 5 μM of DZNep or GSK126 in NCI-H526 (a), NCI-H82 (b), or Daudi (c) cells. Actin or H3 was used as a loading control. Data shown were obtained from averages (± SD) of technical triplicates; significance was determined by two-way ANOVA test followed by Tukey’s correction. The experiments were independently repeated three times with similar results (a–c). d, e Xenograft tumor growth assays were performed using NCI-H526 (d) and NCI-H82 (e) cells upon DZNep or GSK126 treatment. Images of subcutaneous tumors (n = 5 per group) under different treatments are shown. Data shown were obtained from averages (±SD) of technical triplicates; significance was determined by one-way ANOVA test followed by Tukey’s correction (d, e). Source data are provided as a Source data file.

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