Fig. 4: Mesothelial cell activation is driven by receptor tyrosine kinases of the ERBB family.

a Epidermal growth factor receptor (Egfr) and Erbb2 were the main genes encoding for receptor kinases differentially expressed (log count per million, LCPM) over time. b Genes downstream of Egfr and Erbb2 are differentially expressed as well (inhibitory genes are repressed). c Expression of Egfr and Erbb2 genes in mesothelium isolated from germ-free (GF) and specific-pathogen-free (SPF) mice 3 h after sterile injury in combination with bacterial contamination (PB + CLP). Data (a–c) is representative of n = 3 independent animals examined in one experiment. Data are presented as individual values and boxplots (median, first and third quartile) d Peritoneal button, 24 h after injury of Wt1^CreERT2 Rosa26^tdTomato reporter mice that were treated with Tamoxifen 2 weeks prior to experiment. Scale bar 500 µm. Data represents n = 3 animals of 2 independent experiments. e Peritoneal biopsies from healthy mice and from mice 3 days after surgery (PB + CLP). Scale bar 50 µm. Data represents n = 8 animals of 2 independent experiments. Differential expression testing according to the linear modeling as described in the methods section. *P < 0.05, **P < 0.01, ***P < 0.001, n.s. P ≥ 0.05. Exact p-values are provided in Supplementary Data 1. Raw data were deposited at gene expression omnibus (GSE156127).