Fig. 5: EGFR-ligands are produced by bone-marrow derived macrophages and a B-cell subset that are recruited to the wound. | Nature Communications

Fig. 5: EGFR-ligands are produced by bone-marrow derived macrophages and a B-cell subset that are recruited to the wound.

From: Intraperitoneal microbial contamination drives post-surgical peritoneal adhesions by mesothelial EGFR-signaling

Fig. 5

a Illustration of single cell RNA-Sequencing experimental approach. Single cells were isolated from peritoneal buttons (PB) with or without cecal ligation and puncture (CLP). b–d UMAP plots colored by cell cluster (b), expression of epidermal growth factor receptor (Egfr) (c), expression of amphiregulin (Areg) and heparin-binding epidermal growth factor (Hbegf) respectively (d). e Expression of Areg and Hbegf by cell cluster and condition. Differential expression testing with FindMarkers function (Seurat). **P < 0.01, ****P < 0.0001, n.s. P ≥ 0.05. f Schematic illustration of signaling pathways downstream of EGFR. g Western blot stained for phospho-EGFR and the respective downstream molecules illustrated in (f). Primary mesothelial cells were incubated with amphiregulin (AREG) and heparin-binding epidermal growth factor (HB-EGF) with the indicated doses for 20 min. Single-cell data represents a pooled data set of n = 4 mice (n = 2 with CLP, n = 2 injury alone). The immunoblot shown in (g) represents two independent experiments. Raw data were deposited at gene expression omnibus (GSE186658) and Source Data are provided as Source Data File.

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