Fig. 1: Therapeutic activity of anti-BMP1.3 antibody after cardiac damage in vivo. | Nature Communications

Fig. 1: Therapeutic activity of anti-BMP1.3 antibody after cardiac damage in vivo.

From: Bone morphogenetic protein 1.3 inhibition decreases scar formation and supports cardiomyocyte survival after myocardial infarction

Fig. 1

a Quantification of BMP1.3 protein levels in blood of healthy individuals and patients affected by acute myocardial infarction (MI) within 12 h from hospitalization. b Quantification of the expression levels of BMP1.3 normalized over GAPDH in human myocardium of patients without overt cardiac disease (control) or affected by ischemic heart disease (ischemic). c Quantification of BMP1-3 protein levels in the blood of sham-operated or infarcted mice at 24 h after MI. d Quantification of the expression levels of Bmp1.3 normalized over 18 S in the myocardium of sham-operated or infarcted mice at 48 h after MI. e Quantification of the levels of Bmp1.3 mRNA normalized on Actb in different cardiac cell types at 48 h after MI. f Quantification of the ejection fraction in rats treated with isoproterenol (Ip), alone (control) or in combination with anti-BMP1.3 antibody (150 µg/kg). Values for sham animals are also shown for reference. g Quantification of the area covered by collagen in each section. h Representative images of heart sections stained with Sirius red to show collagen deposition upon administration of isoproterenol alone (control) or in combination with anti-BMP1.3 antibody. i Quantification of the area covered by collagen in sections of murine hearts sham-operated or injured by myocardial infarction (MI) and treated with the indicated dose of anti-BMP1.3 antibody (150 or 500 µg/kg). j Representative images of murine heart sections stained with Sirius red to show collagen deposition upon MI and treatment with the indicated dose of anti-BMP1.3 antibody. k Quantification of the ejection fraction at 7, 14, 30, and 60 days after MI. Data in a, b, c, d, e, f, g, i, and k are shown as mean ± s.e.m. Sample number is indicated inside or above each bar. Statistical significance was determined using unpaired, two-sided t-test in a–d, one-way ANOVA, followed by Tukey’s test in e, f, g, i, and two-way ANOVA in k followed by Tukey’s multiple comparison test. Scale bar in h, j is 200 µm and 1 mm, respectively. Source data are provided as a Source Data file.

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