Fig. 7: An assembly of nuclear bodies associates with the active ES in BF T. brucei.
From: An assembly of nuclear bodies associates with the active VSG expression site in African trypanosomes
a Simultaneous visualisation of the ESB, SLAB and NUFIP bodies using SR-SIM imaging of a triple epitope-tagged BF T. brucei cell line. This cell line expresses mNG::RPA2 (RPA2-mNG, green), mCh::SLAP1 (SLAP-mCh, red) and NufB2::6HA (NufB2-HA, magenta). The ESB is indicated with a white arrowhead within a nucleus (dashed line) stained with DAPI (blue). Scale bar = 1 μm. b Quantitation of the distance between the ESB (visualised with RPA2) and the SLAB (visualised with SLAP1) compared with the distance between the ESB and the NUFIP body (visualised with NufB2). Data was of the cell line shown in (a) using SR-SIM imaging. The scatter plot is divided into four quadrants which are coloured according to whether the different bodies in the cells are within 500 nm of each other. Quantitation of the percentage (%) of cells within each coloured quadrant is on the right. Example images of cells from each quadrant are shown in Supplemental Fig. 10. N = 110 cells in G1 (1K1N) from two biological replicates. c Simultaneous visualisation of the Cajal, SLAB and NUFIP bodies using SR-SIM imaging of a triple epitope-tagged cell line. This line expresses mNG::NOP56 (NOP56-mNG, green), mCh::SLAP1 (SLAP-mCh, red) and NufB2::6HA (NufB2-HA, magenta). The NUFIP body is indicated with a light-purple arrowhead within a nucleus (dashed line) stained with DAPI (blue). Scale bar = 1 μm. d Quantitation of the distance of the Cajal body (CB) (visualised with NOP56) and the NUFIP body (visualised with NufB2) compared with the distance between the SLAB (visualised with SLAP1) and the Cajal body. Data were from the cell line shown in (c) using SR-SIM imaging. The scatter plot is divided into quadrants, which are coloured differently according to whether the different bodies in the cells are within 500 nm of each other. Example images of cells from each quadrant are shown in Supplemental Fig. 10. N = 55 cells in G1 (1K1N) from two biological replicates. Source data are provided as a Source Data file.
