Fig. 6: Ddah1 deficiency in osteoblast-lineage cells lacks of the response to mechanical stimulation.
a Representative micro-CT images of trabecular bone and cortical bone in the distal femur (left) of Ddah1f/f and Ddah1prx1 male mice under exercise condition. b Bone volume/total volume (BV/TV) and cortical bone thickness (Ct. Th) of Ddah1f/f and Ddah1prx1 mice. *p < 0.05. ns, no significance. Ddah1f/f control, n = 5. Ddah1f/f exercise, n = 5. Ddah1prx1 control, n = 4. Ddah1prx1 exercise, n = 4. Data are represented as mean values ± SD. c Representative immunofluorescence staining images of trabecular bone in Ddah1f/f and Ddah1prx1 mice treated with running or not. Red for DDAH1. Green for OCN. Scale bar=100 μm. d Quantification analysis of DDAH1 positive or OCN positive cells in bone parameters. ns, no significance. ns, no significance. Ddah1f/f control, n = 5. Ddah1f/f exercise, n = 5. Ddah1prx1 control, n = 5. Ddah1prx1 exercise, n = 5. *p < 0.05. **p < 0.01. ***p < 0.005. Data are represented as mean values ± SD. e Representative double-calcein staining images of trabecular bone in Ddah1f/f and Ddah1prx1 mice treated with running or not. Scale bars = 100 μm. f MAR of double-calcein staining analysis. ns, no significance. Ddah1f/f control, n = 4. Ddah1f/f exercise, n = 4. Ddah1prx1 control, n = 4. Ddah1prx1 exercise, n = 4. *p < 0.05. Data are represented as mean values ± SD. g Results from LC-MS assay for ADMA concentrations of Ddah1prx1 and Ddah1f/f treated with running or not. ns, no significance. Ddah1f/f control, n = 4. Ddah1f/f exercise, n = 4. Ddah1prx1 control, n = 4. Ddah1prx1 exercise, n = 4. *p < 0.05. **p < 0.01. Data are represented as mean values ± SD. The data were analyzed by an unpaired two-tailed Student’s t test in two groups compared. One-way analysis of variance (ANOVA) with post-hoc Tukey’s test was used for experiments with three or more groups.
