Fig. 2: Substrate scope of SptF. | Nature Communications

Fig. 2: Substrate scope of SptF.

From: Molecular insights into the unusually promiscuous and catalytically versatile Fe(II)/α-ketoglutarate-dependent oxygenase SptF

Fig. 2

a–c LC-MS EICs for in vitro assays using preandiloid B (10), preandiloid C (12), terretonin J (13), terretonin C (15), terretonin A (17), and terretonin (19). The + m/z value used for each compound is shown. d Structures of substrates and corresponding products. Products 11, 14, and 16 were isolated from large scale enzymatic reactions, and their structures were determined by NMR. Note that the structures of 18 and 20 were deduced according to the HRMS, UV pattern, and reaction type (Supplementary Figs. 2 and 5).

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