Fig. 3: Oxidation of human steroid hormones by SptF. | Nature Communications

Fig. 3: Oxidation of human steroid hormones by SptF.

From: Molecular insights into the unusually promiscuous and catalytically versatile Fe(II)/α-ketoglutarate-dependent oxygenase SptF

Fig. 3

a LC-MS EICs for in vitro assays using androsterone (21), testosterone (24), and progesterone (28). The + m/z value used for each compound is shown. b Structures of substrates and corresponding products. Products 22, 23, and 25–27 were isolated from large scale enzymatic reactions, and the structures of 22, 23, and 26/27 were determined by NMR, while the structure of 25 was solved by the crystal sponge method. Note that 26 and 27 are interchangeable with each other according to the NMR data. Products 29–31 were not determined due to separation problems, but their structures were deduced to be hydroxylated and/or oxidized compounds, considering their molecular weights (Supplementary Fig. 2).

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