Fig. 3: Dynamics of 1014 F conversion over 10 generations in caged populations.

A Virgin ‘master females’ carrying the y^<CC|pF|> allelic-drive (DR) and Cas9 were seeded among receiver para^L1014F flies at a ratio of 25%:75% (n = 100; N = 3). B Model-predicted dynamics of the y^<CC|pF|> drive element. Mathematical simulations were run using fitted drive parameters and estimated fitness costs. 50 stochastic predictions are plotted in thin lines and their mean in thick lines. Experimental data from 3 independent replicates was overlayed as dotted lines. C Resistance to 50 ppm DDT was tested separately for generations 9–12 (Fig. S6E, F) and averaged. Percent resistance to DDT exposure (assayed by survival) in the presence (drive) or absence (control) of Cas9 combined for all 4 generations is plotted and analyzed using one-way ANOVA followed by Sidak’s multiple comparison tests. D Dynamics of 1014F allelic frequency was modeled separately from the y^<CC|pF|> element. The model predicted a decrease in 1014F levels for both control and drive populations with faster decline in presence of Cas9. E, F The proportion of the 1014F allele was measured by deep sequencing of females and males at generation 1, 5 and 9. The percentage of 1014F alleles in the presence (green dots) or absence (gray dots) of Cas9 was plotted and analyzed using multiple unpaired t tests. Data plotted as mean ± s.e.m. *p < 0.033, **p < 0.0021, ***p < 0.0002, ****p < 0.0001, ns not significant.