Fig. 1: Changes to the astrocyte translatome due to tau and amyloid pathology.

A Schematic illustrating the crossing of MAPT^P301S with the Aldh1l1_eGFP-RPL10a mouse. Astrocyte TRAP-seq performed on MAPT^P301S vs. WT mice (both carrying the Aldh1l1_eGFP-RPL10a allele) at 3 months (B) and 5 months (C) in the spinal cord. Genes significantly induced (red) and repressed (blue) are highlighted (expression cut-off 1FPKM, p values are adjusted for multiple testing by the Benjamini–Hochberg procedure to give a false discovery rate of 5% (p_adj < 0.05)) here and in all RNA-seq analyses; n = 4 mice per genotype. Genes in grey are not significantly changed. See also Supplementary Data 1 and 2. Note that all tests throughout the manuscript are 2-sided. D Sample-by-sample heat map of genes induced (red) or repressed (blue) >1.5-fold at 5 months (p_adj < 0.05). E Log2-fold change of the genes induced > 2-fold in MAPT^P301S at late stage (C) when examined at the early stage (B). t = 11.28, df = 206, p < 1E−15 (Ratio paired t-test of FPKM (WT) vs FPKM (MAPT^P301S)). F Schematic illustrating the crossing of APP/PS1 mouse with the Aldh1l1_eGFP-RPL10a mouse. Astrocyte TRAP-seq performed on APP/PS1 vs. WT mice at 6 months (G) and 12 months (H) in the cortex. Genes significantly induced (red) and repressed (blue) are highlighted (expression cut-off 1FPKM, p_adj < 0.05); n = 4 mice per genotype. See Supplementary Data 3 and 4. I Sample-by-sample heat map of genes induced (red) or repressed (blue) >1.5 fold (p_adj<0.05) at 12 months. J Log2-fold change of the genes induced >2-fold in APP/PS1 at late stage (H) when examined at the early stage (G). t = 14.68, df = 100, p < 1E−15 (Ratio paired t-test of FPKM (WT) vs FPKM (APP/PS1)).