Fig. 2: POC synthesis.
A Conjugating two stereoisomeric azidopeptides to ON-BCNs via strain-promoted alkyne-azide cycloaddition to obtain L,D-POC1-18 and D,D-POC1-15. B Sequence of L-azidopeptide, D-azidopeptide and ONs. C Different linker lengths were employed to furnish the BCN function either on the 5′-end or on the 3′-end of ONs. Natural amino acids are denoted in upper cases while unnatural ones are in lower cases. Thus, Y, V, L, E, S, K, Q, A, G are L-amino acids whereas y, v, l, e, s, k, q, a, g are D-amino acids. A, G, C, and T are natural DNA monomers. Key: The two-letter prefix before POCs: The first letter indicates the chirality of the peptide while the second letter indicates the chirality of the ON.
