Fig. 4: Comparative study of bound % for the L- and D-peptide with different linker lengths.

A Rupture force histograms for the L-peptide D-DNA (left) and D-peptide D-DNA (right) with varying linker length (in number of bonds). B Comparison of bound % for D-peptide D-DNA and L-peptide D-DNA. Data are presented as mean ± SD for n = 4 independent experiments. **P = 0.0085, ***P = 3.9 × 10⁻⁵ for the 21 bonds and P = 2.4 × 10⁻⁵ for the 24–25 bonds, ns: nonsignificant (two tailed unpaired t-test). C Percentage contribution of chirality clash and templated binding effects for the trimeric L-peptide D-DNA with varied linker length (see Methods for calculation). D Rupture force histograms for the L-peptide D-DNA (L,D-POC(2 + 3), 16 bonds) bound with a TFO conjugated with L-peptide (L,D-POC16, 44–45 bonds, left) or with D-peptide (D,D-POC1, 16 bonds, right). N and n depict total numbers of features and distinct molecules, respectively.