Fig. 9: BCR data analysis, part 2.

a IGHV gene (x axis) fractional abundance (y axis) of expanded clones in each sample (label). Colors and shapes represent patients grouped based on the status of the disease and treatment. The results are depicted in boxplots, in which the value for each patient is represented by a dot, the upper and lower bounds represent the 75% and 25% percentiles, respectively. The center bars indicate the medians, and the whiskers denote values up to 1.5 interquartile ranges above the 75% or below the 25% percentiles. Data beyond the end of the whiskers are outliers. N = 10 for Stable and 5 for Progressive. b PCA of IGHV genes (arrows label) based on the fractional abundance from expanded clones of each sample (points label). Colors and shapes represent patients grouped based on treatment. c An example of a B cell clonal lineage tree. Branch lengths represent the expected number of substitutions per codon (see scale bar). d A root-to-tip correlation analysis. Pearson correlation coefficient between divergence and time within each B cell lineage tree (x axis), with corresponding p-values calculated using a permutation test (y axis). The size of each point corresponds to the number of distinct sequence/time point combinations within each clone. Dashed line shows p-value = 0.05. e Number of convergent antibody clusters within each sample. f Convergent antibodies (VDJ) that are specific to patients with stable status and under treatment. Source data are provided as a Source Data file.