Fig. 4: ADNP resolves R-loop structures in vitro.
From: Proximity labeling identifies a repertoire of site-specific R-loop modulators
a R-loop resolution assay with 40 nM and 200 nM ADNP WT and 1 nM R-loop substrates. Positions of duplex and R-loops are shown. For all resolution assays, quantification of 3 independent experiments is shown as mean values ± SEM. Two-sided Student’s t test p-values are shown. b D-loop resolution assay with 40 nM and 200 nM ADNP WT and 1 nM D-loop substrates. Positions of duplex and D-loops are shown. c R-loop resolution assay with 200 nM ADNP WT and 1 nM R-loop substrates without or with ATP as indicated. d R-loop resolution assay with 40 nM and 200 nM ADNPΔHD and 1 nM R-loop substrates. e R-loop resolution assay with 40 nM and 200 nM ADNP ZnF and 1 nM R-loop substrates. f R-loop resolution assay with 40 nM and 200 nM ADNP homeodomain and C terminus and 1 nM R-loop substrates. g R-loop resolution assay with 40 nM and 200 nM ADNP homeodomain alone and 1 nM R-loop substrates. h Summary of R-loop resolution activities of full-length ADNP WT, ADNPΔHD, ADNP ZnF, ADNP HD + C, and the homeodomain alone. Source data underlying (a–g) are provided as a Source data file.
